Mutations of the INK4a locus in squamous cell carcinomas of human skin

被引：46

作者：

Kubo, Y ^{[1
]}

Urano, Y ^{[1
]}

Matsumoto, K ^{[1
]}

Ahsan, K ^{[1
]}

Arase, S ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,SCH MED,DEPT DERMATOL,TOKUSHIMA 770,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 232卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.6217

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The INK4a locus encodes two different, cell cycle-regulating proteins, p16(INK4a) p19(ARF). In this study, we screened mutations in all coding regions of the INK4a locus (exons 1 beta, 1 alpha, 2, and 3) in 21 squamous cell carcinomas (SCCs) of human skin by polymerase chain reaction-single strand conformation polymorphism analysis. Mutations were detected in 3 SCCs in exon 2, which is common to both p16(INK4a) and p19(ARF). These included an in-frame deletion of 21 base pairs from codon 84 to 90, a frameshift mutation of CCC-->TC at codon 75, and a nonsense mutation of CGA-->TGA at codon 80 of the p16(INK4a) gene. These results suggest that inactivation of the INK4a locus has some relevance to the carcinogenesis in at least some of SCCs of human skin. This is the first demonstration of aberrations in the INK4a locus in SCCs of human skin. (C) 1997 Academic Press.

引用

页码：38 / 41

页数：4

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