Rapid kinetic analysis of multichannel records by a simultaneous fit to all dwell-time histograms

被引:62
作者
Csanády, L [1 ]
机构
[1] Rockefeller Univ, Lab Cardiac Membrane Physiol, New York, NY 10021 USA
关键词
D O I
10.1016/S0006-3495(00)76636-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A method is presented for rapidly extracting single-channel transition rate constants from patch-clamp recordings containing signals from several channels. The procedure is based on a simultaneous fit of the observed dwell-time distributions for all conductance levels, using a maximum likelihood approach. This algorithm allows estimation of single-channel rate constants in cases where more advanced methods may be impractical because of their extremely long computational time, A correction is included for the limited time resolution of the recording system, according to theory developed by Roux and Sauve (Biophys. J. 48.149-158, 1985), by accounting for the impact of undetected transitions on the dwell-time distributions, and by introducing an improved practical implementation of a fixed dead time for the case of more than one channel. This feature allows application of the method to noisy data, after filtering, A computer program implementing the method is tested successfully on a variety of simulated multichannel current traces.
引用
收藏
页码:785 / 799
页数:15
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