The molecular diversity of Dscam is functionally required for neuronal wiring specificity in Drosophila

被引：157

作者：

Chen, Brian E. ^{[1
]}

Kondo, Masahiro ^{[1
]}

Garnier, Amélie ^{[1
]}

Watson, Fiona L. ^{[1
]}

Puettmann-Holgado, Roland ^{[1
]}

Lamar, David R. ^{[1
]}

Schmucker, Dietmar ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Neurobiol, Dana Farber Canc Inst,Dept Canc Biol, Boston, MA 02115 USA

来源：

CELL | 2006年 / 125卷 / 03期

关键词：

D O I：

10.1016/j.cell.2006.03.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alternative splicing of Dscam generates an enormous molecular diversity with maximally 38,016 different receptors. Whether this large diversity is required in vivo is currently unclear. We examined the role of Dscam in neuron-target recognition of single mechanosensory neurons, which connect with different target cells through multiple axonal branches' Analysis of Dscam null neurons demonstrated an essential role of Dscam for growth and directed extension of axon branches. Expression of randomly chosen single isoforms could not rescue connectivity but did restore basic axonal extension and rudimentary branching. Moreover, two Dscam alleles were generated that each reduced the maximally possible Dscam diversity to 22,176 isoforms. Reduction of Dscam diversity resulted in specific connectivity defects of mechanosensory neurons. Furthermore, the observed allele-specific phenotypes suggest functional differences among isoforms. Our findings provide evidence that a very large number of structurally unique receptor isoforms is required to ensure fidelity and precision of neuronal connectivity.

引用

页码：607 / 620

页数：14

共 39 条

[1] Ankyrin-based subcellular gradient of neurofascin, an immunoglobulin family protein, directs GABAergic innervation at Purkinje axon initial segment [J].