Gene expression patterns of the liver in response to alcohol: In vivo and in vitro models compared

Two basic models of alcoholic liver disease pathogenesis exist, one in vivo and one in vitro. To justify the in vitro model, evidence is needed to show that it simulates the in vivo model. Therefore, changes in gene expression caused by high ethanol levels were compared using the two models. Many functional pathways were upregulated in both models. These included the insulin signaling pathway, TGF beta signaling pathway, apoptosis, MAPK signaling pathway, writ signaling pathway and apoptosis. Differences were found in the fatty acids synthesis pathway, which was upregulated in vivo; and glycosylation enzymes which were downregulated in vivo. Also, downregulated in vitro were beta oxidation by mitochondria and translation factors. Catalase and superoxide dismutase in mitochondria were upregulated in vitro. These two enzymes have antioxidant effects. In summary, remarkably similar responses to high alcohol levels in the form of changes in gene expression pathways were found in the in vivo and in vitro models tested. (c) 2006 Elsevier Inc. All rights reserved.