Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes

被引:266
作者
Schramm, A
Larsen, LH
Revsbech, NP
Ramsing, NB
Amann, R
Schleifer, KH
机构
[1] TECH UNIV MUNICH, LEHRSTUHL MIKROBIOL, D-80290 MUNICH, GERMANY
[2] AARHUS UNIV, INST BIOL SCI, DEPT MICROBIAL ECOL, DK-8000 AARHUS C, DENMARK
关键词
D O I
10.1128/AEM.62.12.4641-4647.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microprofiles of O-2 and NO3- were measured in nitrifying biofilms from the trickling filter of an aquaculture water recirculation system. By use of a newly developed biosensor for NO3-, it was possible to avoid conventional interference from other ions. Nitrification was restricted to a narrow zone of 50 mu m on the very top of the film. In the same biofilms, the vertical distributions of members of the lithoautotrophic ammonia-oxidizing genus Nitrosomonas and of the nitrite-oxidizing genus Nitrobacter were investigated by applying fluorescence in situ hybridization of whole fixed cells with 16S rRNA-targeted oligonucleotide probes in combination with confocal laser-scanning microscopy. Ammonia oxidizers formed a dense layer of cell clusters in the upper part of the biofilm, whereas the nitrite oxidizers showed less-dense aggregates in close vicinity to the Nitrosomonas clusters, Both species were not restricted to the oxic zone of the biofilm but were also detected in substantially lower numbers in the anoxic layers and even occasionally at the bottom of the biofilm.
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收藏
页码:4641 / 4647
页数:7
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