Comparative proteomics of glycosomes from bloodstream form and procyclic culture form Trypanosoma brucei brucei

被引：116

作者：

Colasante, Claudia ^{[1
]}

Ellis, Margrit ^{[1
]}

Ruppert, Thomas ^{[1
]}

Voncken, Frank ^{[1
]}

机构：

[1] ZMBH, D-69120 Heidelberg, Germany

来源：

PROTEOMICS | 2006年 / 6卷 / 11期

关键词：

glycosome; mass spectrometry; metabolism; peroxisome; Trypanosoma brucei brucei;

D O I：

10.1002/pmic.200500668

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peroxisomes are present in nearly every eukaryotic cell and compartmentalize a wide range of important metabolic processes. Glycosomes of Kinetoplastid parasites are peroxisome-like organelles, characterized by the presence of the glycolytic pathway. The two replicating stages of Trypanosoma brucei brucei, the mammalian bloodstream form (BSF) and the insect (procyclic) form (PCF), undergo considerable adaptations in metabolism when switching between the two different hosts. These adaptations involve also substantial changes in the proteome of the glycosome. Comparative (non-quantitative) analysis of BSF and PCF glycosomes by nano LC-ESI-Q-TOF-MS resulted in the validation of known functional aspects of glycosomes and the identification of novel glycosomal constituents.

引用

页码：3275 / 3293

页数：19

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