A DNA-electrochemical biosensor for screening environmental damage caused by s-triazine derivatives

被引：45

作者：

Oliveira-Brett, AM ^{[1
]}

da Silva, LA ^{[1
]}

机构：

[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2002年 / 373卷 / 08期

关键词：

s-triazine derivatives; interaction with DNA; voltammetric detection; DNA-electrochemical biosensor;

D O I：

10.1007/s00216-002-1259-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrochemical DNA-biosensor has been used to investigate the interactions between DNA and members of a group of ten derivatives of 1,3,5-triazine herbicides: chloro-s-triazines (atrazine, propazine, terbutylazin, and cyanazin), thiomethyl-s-triazines (ametryn, prometryn, terbutryn, and simetryn), and methoxy-s-triazines (prometon and terbumeton). A UV spectrophotometric study of this group of herbicides was also undertaken. Of this group only cyanazin could be oxidized in aqueous solution using a glassy carbon electrode. Use of the electrochemical DNA-biosensor revealed the occurrence of a time-dependent interaction of all the herbicides with DNA, via the appearance of guanine, guanosine, and adenosine oxidation signals that correspond to DNA damage. Adduct formation between the herbicide and the DNA purine bases guanine and adenine is suggested as a mechanism.

引用

页码：717 / 723

页数：7

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