Spatial distribution of GC- and AT-rich DNA sequences within human chromosome territories

被引:40
作者
Tajbakhsh, J
Luz, H
Bornfleth, H
Lampel, S
Cremer, C
Lichter, P
机构
[1] Deutsch Krebsforschungszentrum, Abt Org Komplexer Genome, D-69120 Heidelberg, Germany
[2] Univ Heidelberg, Inst Angew Phys, Heidelberg, Germany
关键词
chromosome territory; GC/AT-rich DNA isochores; FISH; 3D image analysis; subchromosomal organization; nuclear architecture;
D O I
10.1006/excr.1999.4780
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Previous topological analyses of DNA sequence organization in the interphase chromosome mainly focused on the spatial distribution of individual gene copies within chromosome territories. In order to achieve a more comprehensive view into the subchromosomal arrangement of DNA, we isolated the GC-richest/gene-richest fraction (H3 isochores) as well as AT-richest/gene-poorest fraction of human genomic DNA (L1+L2 isochores) and visualized the respective DNA within individual chromosome territories by means of dual-color FISH. Application of confocal laser scanning microscopy and dedicated 3D image analysis software, which differentiated territory subvolumes by peeling shells one voxel in width, revealed a significant difference in the intraterritorial distribution of these two DNA sequence classes. While the H3 isochores were found localized in all subvolumes of the territories at similar frequency, simultaneously detected L1+L2 isochores were observed more to the interior of the same chromosome territories. Thus the GC-rich sequences display a much higher variability in their intraterritorial localization than AT-rich DNA fragments. (C) 2000 Academic Press.
引用
收藏
页码:229 / 237
页数:9
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