An electrophoretic molecular karyotype of a clinical isolate of Aspergillus fumigatus and localization of the MDR-like genes AfuMDR1 and AfuMDR2

被引：11

作者：

Tobin, MB ^{[1
]}

Peery, RB ^{[1
]}

Skatrud, PL ^{[1
]}

机构：

[1] ELI LILLY & CO,LILLY RES LABS,DEPT INFECT DIS,INDIANAPOLIS,IN 46285

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 1997年 / 29卷 / 02期

关键词：

D O I：

10.1016/S0732-8893(97)00103-X

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The molecular karyotype of a clinical isolate of Asperigillus fumigatus (10AF/86/10) was determined by contour-clamped homogeneous electric field gel electrophoresis. Five chromosomal bands were resolved by this method. The resolved chromosomes ranged in size from 1.7 to 4.8 Mb, and together constituted a total genomic size of at least 15.8 Mb. Southern analysis of the separated chromosomes located the position of two MDR-like genes, AfuMDR1 and AfuMDR2, on chromosomes III and IV, respectively. The methods described herein may enable the application of molecular karyotyping of A. fumigatus in epidemiologic surveillance studies. (C) 1997 Elsevier Science Inc.

引用

页码：67 / 71

页数：5

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