Isolation of twelve microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia striiformis f.sp tritici

被引：98

作者：

Enjalbert, J ^{[1
]}

Duan, X

Giraud, T

Vautrin, D

de Vallavielle-Pope, C

Solignac, M

机构：

[1] INRA, Unite Pathol Vegetale & Epidemiol, F-78850 Thiverval Grignon, France

[2] Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100094, Peoples R China

[3] Univ Paris 11, F-91405 Orsay, France

[4] CNRS, F-91198 Gif Sur Yvette, France

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 04期

关键词：

basidiomycota; clonal biotrophic fungus; enriched library; Puccinia striiformis f.sp tritici; yellow rust;

D O I：

10.1046/j.1471-8286.2002.00322.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the characterization of 12 microsatellite markers in the biotrophic fungus Puccinia striiformis f.sp. tritici, responsible for yellow rust on wheat. An enrichment protocol was used to isolate microsatellite loci, and polymorphism was explored with 96 isolates from natural populations collected from several French and Chinese locations. Eight primers (67%) showed cross-amplification when tested with eight isolates of P. triticina.

引用

页码：563 / 565

页数：3

共 6 条

[1] Breakdown of the Yr17 resistance to yellow rust of wheat in northern Europe [J].

Bayles, RA ;

Flath, K ;

Hovmoller, MS ;

de Vallavieille-Pope, C .

AGRONOMIE, 2000, 20 (07) :805-811

[2] Silver staining and recovery of AFLP(TM) amplification products on large denaturing polyacrylamide gels [J].

Chalhoub, BA ;

Thibault, S ;

Laucou, V ;

Rameau, C ;

Hofte, H ;

Cousin, R .

BIOTECHNIQUES, 1997, 22 (02) :216-&

[3] Isolation of eight polymorphic microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Fusarium culmorum [J].

Giraud, T ;

Fournier, E ;

Vautrin, D ;

Solignac, M ;

Vercken, E ;

Bakan, B ;

Brygoo, Y .

MOLECULAR ECOLOGY NOTES, 2002, 2 (02) :121-123

[4] Clonality and long-distance migration of Puccinia striiformis f.sp tritici in north-west Europe [J].

Hovmoller, MS ;

Justesen, AF ;

Brown, JKM .

PLANT PATHOLOGY, 2002, 51 (01) :24-32

[5] The recent history of Puccinia striiformis f.sp tritici in Denmark as revealed by disease incidence and AFLP markers [J].

Justesen, AF ;

Ridout, CJ ;

Hovmoller, MS .

PLANT PATHOLOGY, 2002, 51 (01) :13-23

[6] Support for a stepwise mutation model for pathogen evolution in Australasian Puccinia striiformis f.sp tritici by use of molecular markers [J].

Steele, KA ;

Humphreys, E ;

Wellings, CR ;

Dickinson, MJ .

PLANT PATHOLOGY, 2001, 50 (02) :174-180

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