Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp nov., Thauera aminoaromatica sp nov., and Azoarcus buckelii sp nov.

被引:187
作者
Mechichi, T
Stackebrandt, E
Gad'on, N
Fuchs, G
机构
[1] Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany
[2] Deutsch Sammlung Mikroorganismen & Zellkulturen, D-3300 Braunschweig, Germany
关键词
Thauera; Azoarcus; Thauera phenylacetica sp nov; Thauera aminoaromatica sp nov; Azoarcus buckelii sp nov; nitrate reduction; aromatic compounds; anaerobic degradation;
D O I
10.1007/s00203-002-0422-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
dSix strains of denitrifying bacteria isolated from various oxic and anoxic habitats on different monocyclic aromatic substrates were characterized by sequencing 16S rRNA genes, determining physiological and morphological traits, and DNA-DNA hybridization. According to these criteria, strains S100, S,P and LG356 were identified as members of Thauera aromatica. Strains B5-1 and N5-2 were tentatively affiliated to the species Azoarcus tolulyticus. Strains B4P and S2 were only distantly related to each other and to other described Thauera species. These two strains are proposed as the type strains of two new species, Thauera phenylacetica sp. nov. and Thauera aminoaromatica sp. nov., respectively. By 16S rRNA gene analysis, strain U120 was highly related to the type strains of Azoarcus evansii and Azoarcus anaerobius, whereas corresponding DNA-DNA reassociation values indicated only a low degree of genomic relatedness. Based upon a low DNA similarity value and the presence of distinguishing physiological properties, strain U120 is proposed as the type strain of a new species, Azoarcus buckelii sp. nov. Almost all of the new isolates were obtained with different substrates. The highly varied substrate spectra of the isolates indicates that an even higher diversity of denitrifying bacteria degrading aromatic compounds would be discovered in the different habitats by using a larger spectrum of aromatic substrates for enrichment and isolation.
引用
收藏
页码:26 / 35
页数:10
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