Photophysics and optical switching in green fluorescent protein mutants

被引:112
作者
Creemers, TMH
Lock, AJ
Subramaniam, V
Jovin, TM
Völker, S
机构
[1] Leiden Univ, Huygens Lab, Ctr Study Excited States Mol, NL-2300 RA Leiden, Netherlands
[2] Leiden Univ, Gorlaeus Labs, NL-2300 RA Leiden, Netherlands
[3] Max Planck Inst Biophys Chem, Dept Mol Biol, D-37077 Gottingen, Germany
关键词
D O I
10.1073/pnas.050365997
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not photostable "one-color" systems as previously believed. From our experiments we have further derived the energy-level schemes governing the interconversion among the three forms. These results have significant implications for the molecular and cell biological applications of the green fluorescent protein family; for example, in fluorescence resonant energy transfer experiments, a change in "color" on irradiation may not necessarily he due to energy transfer but can also arise from a photo-induced conversion between conformers of the excited species.
引用
收藏
页码:2974 / 2978
页数:5
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