The poly dA helix: a new structural motif for high performance DNA-based molecular switches

被引：122

作者：

Chakraborty, Saikat ^{[1
]}

Sharma, Suruchi ^{[1
]}

Maiti, Prabal K. ^{[2
]}

Krishnan, Yamuna ^{[1
]}

机构：

[1] GKVK, TIFR, Natl Ctr Biol Sci, Bangalore 560065, Karnataka, India

[2] Indian Inst Sci, Ctr Condensed Matter Theory, Dept Phys, Bangalore 560012, Karnataka, India

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 09期

关键词：

RAY FIBER DIFFRACTION; HYBRID I-MOTIF; CONFORMATIONAL SWITCH; MAGNETIC-RESONANCE; POLYADENYLIC-ACID; STRANDED-DNA; SINGLE; DUPLEX; RNA; POLYNUCLEOTIDES;

D O I：

10.1093/nar/gkp133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a pH-dependent conformational transition in short, defined homopolymeric deoxyadenosines (dA(15)) from a single helical structure with stacked nucleobases at neutral pH to a double-helical, parallel-stranded duplex held together by AH-HA base pairs at acidic pH. Using native PAGE, 2D NMR, circular dichroism (CD) and fluorescence spectroscopy, we have characterized the two different pH dependent forms of dA(15). The pH-triggered transition between the two defined helical forms of dA(15) is characterized by CD and fluorescence. The kinetics of this conformational switch is found to occur on a millisecond time scale. This robust, highly reversible, pH-induced transition between the two well-defined structured states of dA(15) represents a new molecular building block for the construction of quick-response, pH-switchable architectures in structural DNA nanotechnology.

引用

页码：2810 / 2817

页数：8

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