Coexistence of Copy Number Increases of c-Myc, ZNF217, CCND1, ErbB1 and ErbB2 in Ovarian Cancers

Background: We selected 5 oncogenes with well-established roles in carcinogenesis - CCND1, ErbB1, ErbB2, c-myc and ZNF217 - to investigate the coexistence of their copy imbalances in relation to the clinico-pathological characteristics of ovarian tumors. Materials and Methods: Fluorescence in situ hybridization for the 5 genes was applied to a preexisting tissue microarray. 38 ovarian tumors were successfully analyzed for copy number changes of the 5 genes. Results: At least one of these oncogenes was gained/amplified in 27 out of 38 tumors (71.1%). We report the highest frequency of c-myc genetic gain/amplification since it affected 42.1% of the ovarian tumors. We observed sequential involvement of copy number alterations of the other genes in the presence of c-myc disruption. The incidence of copy number changes of the 5 oncogenes - both single and combinatorial was higher in high-grade tumors. All double aberrations in the serous group comprised c-myc and ZNF217 copy number increases. Conclusions: Our results revealed a combination between copy number increases of c-myc and ZNF217, associated with serous histology. The data from this combined analysis of the 5 oncogenes could be used as a basis in considering the combined approach in molecular-based therapy of ovarian cancer.