Spectral and electrochemical detection of protonated triplex formation by a small-molecule anticancer agent

被引：18

作者：

Feng, Lingyan ^{[1
]}

Li, Xi ^{[1
]}

Peng, Yinghua ^{[1
]}

Geng, Jie ^{[1
]}

Ren, Jinsong ^{[1
]}

Qu, Xiaogang ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resource Utilizat, Div Biol Inorgan Chem,Grad Sch, Changchun 130022, Jilin, Peoples R China

来源：

CHEMICAL PHYSICS LETTERS | 2009年 / 480卷 / 4-6期

关键词：

DNA FLANKING SEQUENCE; STRUCTURAL SELECTIVITY; THERMODYNAMIC ANALYSIS; BASE-STACKING; CYTOCHROME-C; B-FORM; BINDING; DUPLEX; STABILIZATION; CORALYNE;

D O I：

10.1016/j.cplett.2009.09.025

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Triplex helical formation has been the focus of considerable interest because of possible applications in developing new molecular biology tools as well as therapeutic agents and the possible relevance of H-DNA structures in biology system. We report here that a small-molecule anticancer agent, coralyne, has binding preference to the less stable protonated triplex d(C+-T)(6):d(A-G)(6).d(C-T)(6) over duplex d(A-G)(6).d(C-T)(6) and shows different spectral and electrochemical characteristics when binding to triplex and duplex DNA, indicating that electrochemical technique can detect the less stable protonated triplex formation. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：309 / 312

页数：4

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