Evaluation of chemiluminescent estradiol conjugates by using a surface plasmon resonance detector

被引：26

作者：

Adamczyk, M ^{[1
]}

Chen, YY ^{[1
]}

Gebler, JC ^{[1
]}

Johnson, DD ^{[1
]}

Mattingly, PG ^{[1
]}

Moore, JA ^{[1
]}

Reddy, RE ^{[1
]}

Wu, J ^{[1
]}

Yu, ZG ^{[1
]}

机构：

[1] Abbott Labs, Div Diagnost, Dept Chem, Abbott Pk, IL 60064 USA

来源：

STEROIDS | 2000年 / 65卷 / 06期

关键词：

steroid; 17; beta-estradiol; chemiluminescent probes; anti-estradiol mAb; BIAcore; affinity;

D O I：

10.1016/S0039-128X(00)00091-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of chemiluminescent 17 beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K-D) for the interaction of an anti-E-2 Fab fragment for the probes in solution were evaluated using a single E-2-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The results show the antibody fragment binds all chemiluminescent conjugates tested with high affinity showing only minor preferences for site of substitution (C6 versus C7), stereochemistry (alpha versus beta), or linker moiety. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：295 / 303

页数：9

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