Histochemical visualization of phosphatase released by arbuscular mycorrhizal fungi in soil

被引:34
作者
Feng, G
Su, YB
Li, XL [1 ]
Wang, H
Zhang, FS
Tang, CX
Rengel, Z
机构
[1] China Agr Univ, Dept Plant Nutr, Beijing 100094, Peoples R China
[2] Univ Western Australia, Nedlands, WA 6907, Australia
关键词
arbuscular mycorrhizal hyphae; in situ histochemical localization; phosphatase activity; soil organic phosphorus;
D O I
10.1081/PLN-120003932
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A histochemical procedure was developed to visualize the excretion of phosphatase by arbuscular mycorrhizal (AM) fungus in soil. Red clover (Trifolium pratense L.) plants were grown in three-compartment pots under controlled conditions. Each pot was separated into three compartments by 30-mum nylon mesh, the central one for plant growth and two outer ones for growth of arbuscular mycorrhizal hyphae. Plants in the central compartments were either uninoculated or inoculated with AM fungus (Glomus mosseae). Plants were harvested after 9 weeks. The activities of acid and alkaline phosphatases and organic P depletion were measured at different distances from the central compartment. The acid phosphatase released by AM hyphae in soil was also assessed in situ using transmission electron microscopy. The results showed that AM hyphae increased the activities of acid and alkaline phosphatases and decreased the concentration of soil organic phosphorus in the hyphal compartments. Acid phosphatase in soil was observed around the AM hyphae by an in situ histochemical method. To our knowledge, this is the first direct evidence that AM fungus excretes phosphatases to soil.
引用
收藏
页码:969 / 980
页数:12
相关论文
共 27 条
[1]  
[Anonymous], 1978, SOIL ENZYMES
[2]  
Dalal R. C., 1977, Advances in Agronomy, V29, P83
[3]   PHOSPHATASE-ACTIVITY ASSOCIATED WITH THE ROOTS AND THE RHIZOSPHERE OF PLANTS INFECTED WITH VESICULAR ARBUSCULAR MYCORRHIZAL FUNGI [J].
DODD, JC ;
BURTON, CC ;
BURNS, RG ;
JEFFRIES, P .
NEW PHYTOLOGIST, 1987, 107 (01) :163-172
[4]   COMPARISON OF PHOSPHATASE LOCALIZATION IN THE INTRARADICAL HYPHAE OF ARBUSCULAR MYCORRHIZAL FUNGI, GLOMUS SPP AND GIGASPORA SPP [J].
EZAWA, T ;
SAITO, M ;
YOSHIDA, T .
PLANT AND SOIL, 1995, 176 (01) :57-63
[5]   AN EVALUATION OF TECHNIQUES FOR MEASURING VESICULAR ARBUSCULAR MYCORRHIZAL INFECTION IN ROOTS [J].
GIOVANNETTI, M ;
MOSSE, B .
NEW PHYTOLOGIST, 1980, 84 (03) :489-500
[6]   AN IMPROVED HISTOCHEMICAL TECHNIC FOR ACID PHOSPHATASE [J].
GOMORI, G .
STAIN TECHNOLOGY, 1950, 25 (02) :81-85
[7]  
GROSSRAU ZL, 1979, ENZYME HISTOCHEMISTR, P58
[8]  
Jackson ML, 1967, Soil Chemical Analysis, P134
[9]   UPTAKE OF P-32 FROM LABELED ORGANIC-MATTER BY MYCORRHIZAL AND NONMYCORRHIZAL SUBTERRANEAN CLOVER (TRIFOLIUM-SUBTERRANEUM L) [J].
JONER, EJ ;
JAKOBSEN, I .
PLANT AND SOIL, 1995, 172 (02) :221-227
[10]   Phosphatase activity of external hyphae of two arbuscular mycorrhizal fungi [J].
Joner, EJ ;
Johansen, A .
MYCOLOGICAL RESEARCH, 2000, 104 :81-86