Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development

被引：23

作者：

Imamura, T

Neildez, TMA

Thenevin, C

Paldi, A

机构：

[1] Inst Jacques Monod, INSERM E367, F-75005 Paris, France

[2] Ecole Prat Hautes Etud, F-75005 Paris, France

[3] GENETHON, F-91002 Evry, France

[4] Natl Inst Basic Biol, Dept Regulat Biol, Div Behav & Neurobiol, Okazaki 4448585, Japan

来源：

BMC MOLECULAR BIOLOGY | 2004年 / 5卷

关键词：

D O I：

10.1186/1471-2199-5-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl) ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl) ation is involved in the epigenetic chromatin remodeling in the zygote.

引用

页数：11

共 24 条

[1]

Adenot PG, 1997, DEVELOPMENT, V124, P4615

[2]

Arney KL, 2002, INT J DEV BIOL, V46, P317

[3] Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions [J].