Inhibition of phosphatases and increased Ca2+ channel activity by inositol hexakisphosphate

被引：112

作者：

Larsson, O

Barker, CJ

Sjoholm, A

Carlqvist, H

Michell, RH

Bertorello, A

Nilsson, T

Honkanen, RE

Mayr, GW

Zwiller, J

Berggren, PO

机构：

[1] KAROLINSKA INST,DEPT MOL MED,ROLF LUFT CTR DIABET RES,S-17176 STOCKHOLM,SWEDEN

[2] ASTRA PAIN CONTROL AB,NOVUM UNIT,S-14157 HUDDINGE,SWEDEN

[3] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

[4] UNIV S ALABAMA,COLL MED,DEPT BIOCHEM,MOBILE,AL 36688

[5] UNIV HAMBURG,HOSP EPPENDORF,INST PHYSIOL CHEM,D-20246 HAMBURG,GERMANY

[6] INSERM,U338,F-67084 STRASBOURG,FRANCE

来源：

SCIENCE | 1997年 / 278卷 / 5337期

关键词：

D O I：

10.1126/science.278.5337.471

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Inositol hexakisphosphate (InsP(6)), the dominant inositol phosphate in insulin-secreting pancreatic beta cells, inhibited the serine-threonine protein phosphatases type 1, type 2A, and type 3 in a concentration-dependent manner. The activity of voltage-gated L-type calcium channels is increased in cells treated with inhibitors of serine-threonine protein phosphatases. Thus, the increased calcium channel activity obtained in the presence of InsP(6) might result from the inhibition of phosphatase activity. Glucose elicited a transient increase in InsP(6) concentration, which indicates that this inositol polyphosphate may modulate calcium influx over the plasma membrane and serve as a signal in the pancreatic beta cell stimulus-secretion coupling.

引用

页码：471 / 474

页数：4

共 34 条

[1] ACTIVATION OF PROTEIN-KINASES AND INHIBITION OF PROTEIN PHOSPHATASES PLAY A CENTRAL ROLE IN THE REGULATION OF EXOCYTOSIS IN MOUSE PANCREATIC BETA-CELLS [J].