Variation in the safety of induced pluripotent stem cell lines

被引：653

作者：

Miura, Kyoko ^{[1
,2
,3
,4
]}

Okada, Yohei ^{[1
,5
]}

Aoi, Takashi ^{[2
,4
]}

Okada, Aki ^{[2
]}

Takahashi, Kazutoshi ^{[2
,4
]}

Okita, Keisuke ^{[2
,4
]}

Nakagawa, Masato ^{[2
,3
,4
]}

Koyanagi, Michiyo ^{[2
,4
]}

Tanabe, Koji ^{[2
,3
,4
]}

Ohnuki, Mari ^{[2
,3
,4
]}

Ogawa, Daisuke ^{[1
]}

Ikeda, Eiji ^{[6
]}

Okano, Hideyuki ^{[1
]}

Yamanaka, Shinya ^{[2
,3
,4
,7
]}

机构：

[1] Keio Univ, Sch Med, Dept Physiol, Tokyo 160, Japan

[2] Kyoto Univ, Ctr iPS Cell Res & Applicat CiRA, Kyoto, Japan

[3] Kyoto Univ, Inst Frontier Med Sci, Dept Stem Cell Biol, Kyoto, Japan

[4] Japan Sci & Technol Agcy, Yamanaka iPS Cell Special Project, Kawaguchi, Saitama, Japan

[5] Keio Univ, Sch Med, Kanrinmaru Project, Tokyo 160, Japan

[6] Keio Univ, Sch Med, Dept Pathol, Tokyo 160, Japan

[7] Gladstone Inst Cardiovasc Dis, San Francisco, CA USA

来源：

NATURE BIOTECHNOLOGY | 2009年 / 27卷 / 08期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

GENERATION; FIBROBLASTS; MOUSE; MYC;

D O I：

10.1038/nbt.1554

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We evaluated the teratoma-forming propensity of secondary neurospheres (SNS) generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways. Teratoma-formation of SNS from embryonic fibroblast-derived iPS cells was similar to that of SNS from embryonic stem (ES) cells. In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells.

引用

页码：743 / 745

页数：3

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