A novel experiment for the quantitative measurement of CSA(1HN)/CSA(15N) cross-correlated relaxation in 15N-labeled proteins

被引：23

作者：

Tessari, M ^{[1
]}

Vuister, GW ^{[1
]}

机构：

[1] Univ Nijmegen, Nijnmegen SON Res Ctr, Dept Biophys Chem, Nijmegen, Netherlands

来源：

JOURNAL OF BIOMOLECULAR NMR | 2000年 / 16卷 / 02期

关键词：

cross-correlation; CSA; EH2; Eps15; relaxation;

D O I：

10.1023/A:1008347012429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An experiment is presented which allows for the quantitative measurement of the relaxation interference between the H-1(N) CSA and N-15 CSA interactions in N-15 labeled proteins. A constant-time buildup scheme is used to measure the differential relaxation rate, eta, between double-quantum (DQ) and zero-quantum (ZQ) H-1(N)-N-15 coherences. The CSA/CSA experiment was recorded at three different B-o field strengths. The CSA(H-1(N))/CSA(N-15) cross-correlation rate was obtained from the linear fit of the measured rate, eta, versus B-o(2) for 77 residues of the EH2 domain from mouse Eps15.

引用

页码：171 / 174

页数：4

共 13 条

[1] BAND-SELECTIVE RADIOFREQUENCY PULSES [J].

GEEN, H ;

FREEMAN, R .

JOURNAL OF MAGNETIC RESONANCE, 1991, 93 (01) :93-141

[2]

GERALD R, 1993, J AM CHEM SOC, V115, P772

[3] CROSS-CORRELATION EFFECTS IN THE TRANSVERSE RELAXATION OF MULTIPLE-QUANTUM TRANSITIONS OF HETERONUCLEAR SPIN SYSTEMS [J].

KONRAT, R ;

STERK, H .

CHEMICAL PHYSICS LETTERS, 1993, 203 (01) :75-80

[4] Longitudinal and transverse 1H-15N dipolar 15N chemical shift anisotropy relaxation interference:: Unambiguous determination of rotational diffusion tensors and chemical exchange effects in biological macromolecules [J].

Kroenke, CD ;

Loria, JP ;

Lee, LK ;

Rance, M ;

Palmer, AG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (31) :7905-7915

[5] Effect of remote cross correlations on transverse relaxation [J].

Kumar, P ;

Kumar, A .

JOURNAL OF MAGNETIC RESONANCE SERIES A, 1996, 119 (01) :29-37

[6] Influence of cross-correlation between the chemical shift anisotropies of pairs of nuclei on multiple-quantum relaxation rates in macromolecules [J].

Norwood, TJ ;

Tillett, ML ;

Lian, LY .

CHEMICAL PHYSICS LETTERS, 1999, 300 (3-4) :429-434

[7] Direct measurement of angles between bond vectors in high-resolution NMR [J].

Reif, B ;

Hennig, M ;

Griesinger, C .

SCIENCE, 1997, 276 (5316) :1230-1233

[8] GRADIENT-TAILORED WATER SUPPRESSION FOR H-1-N-15 HSQC EXPERIMENTS OPTIMIZED TO RETAIN FULL SENSITIVITY [J].

SKLENAR, V ;

PIOTTO, M ;

LEPPIK, R ;

SAUDEK, V .

JOURNAL OF MAGNETIC RESONANCE SERIES A, 1993, 102 (02) :241-245

[9] Determination of amide proton CSA in N-15-labeled proteins using H-1 CSA/N-15-H-1 dipolar and N-15 CSA/N-15-H-1 dipolar cross-correlation rates [J].

Tessari, M ;

Mulder, FAA ;

Boelens, R ;

Vuister, GW .

JOURNAL OF MAGNETIC RESONANCE, 1997, 127 (01) :128-133

[10] Quantitative measurement of relaxation interference effects between H-1(N) CSA and H-1-N-15 dipolar interaction: Correlation with secondary structure [J].

Tessari, M ;

Vis, H ;

Boelens, R ;

Kaptein, R ;

Vuister, GW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (38) :8985-8990

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