Detection and immunolocalization of macrophage migration inhibitory factor in rat iris and ciliary epithelium

被引：18

作者：

Matsuda, A ^{[1
]}

Kotake, S ^{[1
]}

Tagawa, Y ^{[1
]}

Matsuda, H ^{[1
]}

Nishihira, J ^{[1
]}

机构：

[1] HOKKAIDO UNIV,SCH MED,CENT RES INST,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

IMMUNOLOGY LETTERS | 1996年 / 53卷 / 01期

关键词：

macrophage migration inhibitory factor; ciliary epithelium; immunohistochemistry; western blot;

D O I：

10.1016/0165-2478(96)02600-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To elucidate the role of macrophage migration inhibitory factor (MIF) in ocular inflammation, we examined the localization of MIF in the normal anterior uveal tract of rats. Immunohistochemistry using an anti-MIF antibody revealed that MIF was present in non-pigment epithelial cells of the ciliary body and the epithelial cells of the iris. Western blot analysis of these tissues showed a single band specific for MIF protein. The expression of MIF mRNA in these tissues was further confirmed by reverse transcription-polymerase chain reaction. Since MIF is known to be a potent proinflammatory cytokine, identification bf the protein in iris and ciliary epithelial cells suggests the possibility that it may play an important role in ocular inflammation.

引用

页码：1 / 5

页数：5

共 25 条

[1] MIF IS A PITUITARY-DERIVED CYTOKINE THAT POTENTIATES LETHAL ENDOTOXEMIA [J].

BERNHAGEN, J ;

CALANDRA, T ;

MITCHELL, RA ;

MARTIN, SB ;

TRACEY, KJ ;

VOELTER, W ;

MANOGUE, KR ;

CERAMI, A ;

BUCALA, R .

NATURE, 1993, 365 (6448) :756-759

[2] An essential role for macrophage migration inhibitory factor in the tuberculin delayed-type hypersensitivity reaction [J].

Bernhagen, J ;

Bacher, M ;

Calandra, T ;

Metz, CN ;

Doty, SB ;

Donnelly, T ;

Bucala, R .

JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (01) :277-282

[3]

Bernhagen Juergen, 1994, Trends in Microbiology, V2, P198, DOI 10.1016/0966-842X(94)90111-H

[4] MECHANISM OF A REACTION IN VITRO ASSOCIATED WITH DELAYED-TYPE HYPERSENSITIVITY [J].

BLOOM, BR ;

BENNETT, B .

SCIENCE, 1966, 153 (3731) :80-&

[5] MACROPHAGE IS AN IMPORTANT AND PREVIOUSLY UNRECOGNIZED SOURCE OF MACROPHAGE-MIGRATION INHIBITORY FACTOR [J].

CALANDRA, T ;

BERNHAGEN, J ;

MITCHELL, RA ;

BUCALA, R .

JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (06) :1895-1902

[6] MIF AS A GLUCOCORTICOID-INDUCED MODULATOR OF CYTOKINE PRODUCTION [J].

CALANDRA, T ;

BERNHAGEN, J ;

METZ, CN ;

SPIEGEL, LA ;

BACHER, M ;

DONNELLY, T ;

CERAMI, A ;

BUCALA, R .

NATURE, 1995, 377 (6544) :68-71

[7]

CAPLIOLI J, 1992, ADLERS PHYSL EYE, P228

[8]

KINSLEY TL, 1994, INVEST OPHTH VIS SCI, V35, P3711

[9] Identification and immunohistochemical localization of macrophage migration inhibitory factor in human cornea [J].

Matsuda, A ;

Tagawa, Y ;

Matsuda, H ;

Nishihira, J .

FEBS LETTERS, 1996, 385 (03) :225-228

[10]

NISHIHIRA J, 1993, BIOCHEM MOL BIOL INT, V31, P841

← 1 2 3 →