Type of SCN5A mutation determines clinical severity and degree of conduction slowing in loss-of-function sodium channelopathies

BACKGROUND Patients carrying toss-of-function SCN5A mutations linked to Brugada syndrome (BrS) or progressive cardiac conduction disease (PCCD) are at risk of sudden cardiac death at a young age. The penetrance and expressivity of the disease are highly variable, and new toots for risk stratification are needed. OBJECTIVES We aimed to establish whether the type of SCN5A mutation correlates with the clinical and electrocardiographic phenotype. METHODS We studied BrS or PCCD probands and their relatives who carded a SCN5A mutation. Mutations were divided into 2 main groups: missense mutations (M) or mutations leading to premature truncation of the protein (T). The M group was subdivided according to available biophysical properties: M mutations with : 90% (M-inactive) or > 90% (M-inactive) peak I-Na reduction were analyzed separately. RESULTS The study group was composed of 147 individuals with 32 different mutations. No differences in age and sex distribution were found between the groups. Subjects carrying a T mutation had significantly more syncopes than those with an M-active mutation (19 of 75 versus 2 of 35, P = .03). Also, mutations associated with drastic peak I-Na reduction (T and M-inactive mutants) had a significantly longer PR interval, compared with M-active mutations. All other electrocardiographic parameters were comparable. After drug provocation testing, both PR and QRS intervals were significantly longer in the T and M-inactive groups than in the M-active group. CONCLUSION In Loss-of-function SCN5A channelopathies, patients carrying T and M-inactive mutations develop a more severe phenotype than those with M-active mutations. This is associated with more severe conduction disorders. This is the first time that genetic data are proposed for risk stratification in BrS.