Construction and application of a promoter-trapping vector with methyl parathion hydrolase gene mpd as the reporter

被引：19

作者：

Cui, ZL ^{[1
]}

Zhang, XZ ^{[1
]}

Zhang, ZH ^{[1
]}

Li, SP ^{[1
]}

机构：

[1] Nanjing Agr Univ, Dept Microbiol, MOA Key Lab Microbiol Engn Agr Environm, Nanjing 210095, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 14期

关键词：

Bacillus subtilis; gene expression; genomic promoter library; methyl parathion hydrolase; promoter-trapping vector;

D O I：

10.1023/B:BILE.0000035481.03854.41

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A facilitative and efficient promoter-trapping vector, pUC-mpd, was constructed with the promoterless methyl parathion hydrolase gene as the reporter. This reporter gene is easily used to clone promoters with different promoting strength on selective plates. Promoter regions of the ytkA and ywoF genes with strong promoting and signal peptide functions were cloned from the Bacillus subtilis 168 genomic promoter library with this vector.

引用

页码：1115 / 1118

页数：4

共 12 条

[1] A promoter-trap vector for clock-controlled genes in the cyanobacterium Synechocystis sp PCC 6803 [J].

Aoki, S ;

Kondo, T ;

Ishiura, M .

JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 49 (03) :265-274

[2]

BURKHOLDER PR, 1947, AM J BOT, V33, P345

[3]

CHANG S, 1987, METHOD ENZYMOL, V153, P507

[4]

Cui ZL, 2001, APPL ENVIRON MICROB, V67, P4922

[5]

DOI RH, 1983, RECOMBINANT DNA TECH, P162

[6] A vector for promoter trapping in Bacillus cereus [J].

Dunn, AK ;

Handelsman, J .

GENE, 1999, 226 (02) :297-305

[7] Effects of ultraviolet light irradiation in biotreatment of organophosphates [J].

Hung, SC ;

Liao, JC .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1996, 56 (01) :37-47

[8] The complete genome sequence of the Gram-positive bacterium Bacillus subtilis [J].

Kunst, F ;

Ogasawara, N ;

Moszer, I ;

Albertini, AM ;

Alloni, G ;

Azevedo, V ;

Bertero, MG ;

Bessieres, P ;

Bolotin, A ;

Borchert, S ;

Borriss, R ;

Boursier, L ;

Brans, A ;

Braun, M ;

Brignell, SC ;

Bron, S ;

Brouillet, S ;

Bruschi, CV ;

Caldwell, B ;

Capuano, V ;

Carter, NM ;

Choi, SK ;

Codani, JJ ;

Connerton, IF ;

Cummings, NJ ;

Daniel, RA ;

Denizot, F ;

Devine, KM ;

Dusterhoft, A ;

Ehrlich, SD ;

Emmerson, PT ;

Entian, KD ;

Errington, J ;

Fabret, C ;

Ferrari, E ;

Foulger, D ;

Fritz, C ;

Fujita, M ;

Fujita, Y ;

Fuma, S ;

Galizzi, A ;

Galleron, N ;

Ghim, SY ;

Glaser, P ;

Goffeau, A ;

Golightly, EJ ;

Grandi, G ;

Guiseppi, G ;

Guy, BJ ;

Haga, K .

NATURE, 1997, 390 (6657) :249-256

[9] ENZYMATIC DETOXIFICATION OF WASTE ORGANO-PHOSPHATE PESTICIDES [J].

MUNNECKE, DM .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1980, 28 (01) :105-111

[10]

Sambrook J, 1989, MOL CLONING LAB MANU

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