Simultaneous determination of gemcitabine di- and triphosphate in human blood mononuclear and cancer cells by RP-HPLC and UV detection

被引：30

作者：

Losa, R. ^{[1
]}

Sierra, M. I. ^{[1
]}

Gion, M. O. ^{[1
]}

Esteban, E. ^{[1
]}

Buesa, J. M. ^{[1
]}

机构：

[1] Hosp Cent Asturias, Lab Med Oncol, IUOPA, Oviedo 33006, Asturias, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 840卷 / 01期

关键词：

dFdCTP; dFdCDP; reverse-phase HPLC; peripheral blood mononuclear cells; gemcitabine;

D O I：

10.1016/j.jchromb.2006.04.036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse-phase HPLC method based on ion-pair formation with UV detection was set up for the simultaneous determination of gemcitabine diphosphate(dFdCDP) and triphosphate (dFdCTP) in human cells. The separation was achieved on a Tracer Excel ODSA column (100 mm x 4.6 mm i.d., 3 mu m particle size) at room temperature. Nine nucleotides were separated by isocratic elution in 26 min. Accuracy, linearity, sensitivity and precision studies for dFdCDP, dFdCTP, adenosine diphosphate (ADP) and triphosphate (ATP) validated this method. This assay was used to provide data from gemcitabine treated patients and in vitro grown human cancer cells. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：44 / 49

页数：6

共 21 条

[1] Validation of an analysis method for 4-amino-3-hydroxybutyric acid by reversed-phase liquid chromatography [J].

Candela, M ;

Ruiz, A ;

Feo, FJ .

JOURNAL OF CHROMATOGRAPHY A, 2000, 890 (02) :273-280

[2] LEVELS AND VARIABILITY OF PURINE NUCLEOTIDES IN NORMAL HUMAN-LYMPHOCYTES [J].

CARLUCCI, F ;

TABUCCHI, A ;

CONSOLMAGNO, E ;

TAGLIAFERRI, P ;

PORCELLI, B ;

MARINELLO, E ;

LEONCINI, R ;

PAGANI, R .

BIOMEDICINE & PHARMACOTHERAPY, 1992, 46 (2-3) :109-114

[3] Determination of fifteen nucleotides in cultured human mononuclear blood and umbilical vein endothelial cells by solvent generated ion-pair chromatography [J].

Cichna, M ;

Raab, A ;

Daxecker, H ;

Griesmacher, A ;

Müller, MM ;

Markl, R .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 787 (02) :381-391

[4] Simultaneous determination of deoxyribonucleoside in the presence of ribonucleoside triphosphates in human carcinoma cells by high-performance liquid chromatography [J].

Decosterd, LA ;

Cottin, E ;

Chen, X ;

Lejeune, F ;

Mirimanoff, RO ;

Biollaz, J ;

Coucke, PA .

ANALYTICAL BIOCHEMISTRY, 1999, 270 (01) :59-68

[5] VALIDATED ASSAYS FOR THE DETERMINATION OF GEMCITABINE IN HUMAN PLASMA AND URINE USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION [J].

FREEMAN, KB ;

ANLIKER, S ;

HAMILTON, M ;

OSBORNE, D ;

DHAHIR, PH ;

NELSON, R ;

ALLERHEILIGEN, SRB .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 665 (01) :171-181

[6]

GRANHAM KA, 2000, NUCLEOS NUCLEOT, V19, P415

[7]

HEINEMANN V, 1992, CANCER RES, V52, P533

[8]

HEINEMANN V, 1988, CANCER RES, V48, P4024

[9]

HUANG P, 1991, CANCER RES, V51, P6110

[10] Phosphorylation of pyrimidine deoxynucleoside analog Diphosphates - Selective phosphorylation of L-nucleoside analog diphosphates by 3-phosphoglycerate kinase [J].

Krishnan, P ;

Fu, Q ;

Lam, W ;

Liou, JY ;

Dutschman, G ;

Cheng, YC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (07) :5453-5459

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