Effect of cigarette smoking on haem-oxygenase expression in alveolar macrophages

被引：8

作者：

Atzori, L

Caramori, G

Lim, S

Jazrawi, E

Donnelly, L

Adcock, I

Barnes, PJ

Chung, KF

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Sch Med, Natl Heart & Lung Inst, Dept Thorac Med, London SW3 6LY, England

[2] Univ Cagliari, Dept Toxicol, Sect Oncol & Mol Pathol, Cagliari, Italy

来源：

RESPIRATORY MEDICINE | 2004年 / 98卷 / 06期

关键词：

HO-1; HO-2; carbon monoxide; smoking;

D O I：

10.1016/j.rmed.2003.11.007

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We investigated the effect. of chronic cigarette smoking on the expression of haem-oxygenase (HO)-1 and HO-2. Normal subjects and asymptomatic young current smokers with normal lung function tests underwent bronchoalveolar lavage for recovery of macrophages. Reverse transcription/polymerase chain reaction (RTPCR) analysis showed no significant difference in HO-1 and HO-2 mRNA expression between the two groups. On the other hand, Western blot analysis showed a significant (P<0.05) reduction of HO-2 protein, but not of HO-1, in alveolar macrophages from smokers compared to normal. There was no significant differences by immunocytochemistry for HO-1 and HO-2 expression between the groups. We concluded that HO-2 expression is reduced in alveolar macrophages of smokers, possibly due to the oxidative stress of cigarette smoke. This may in turn lead to reduced protection against further oxidative insults. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：530 / 535

页数：6

共 21 条

[1] Medical progress: Chronic obstructive pulmonary disease. [J].

Barnes, PJ .

NEW ENGLAND JOURNAL OF MEDICINE, 2000, 343 (04) :269-280

[2] REACTIVE OXYGEN SPECIES AND AIRWAY INFLAMMATION [J].

BARNES, PJ .

FREE RADICAL BIOLOGY AND MEDICINE, 1990, 9 (03) :235-243

[3] Carbon monoxide attenuates aero allergen-induced inflammation in mice [J].

Chapman, JT ;

Otterbein, LE ;

Elias, JA ;

Choi, AMK .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2001, 281 (01) :L209-L216

[4] Heme oxygenase-mediated resistance to oxygen toxicity in hamster fibroblasts [J].

Dennery, PA ;

Sridhar, KJ ;

Lee, CS ;

Wong, HE ;

Shokoohi, V ;

Rodgers, PA ;

Spitz, DR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (23) :14937-14942

[5] Oxygen toxicity and iron accumulation in the lungs of mice lacking heme oxygenase-2 [J].

Dennery, PA ;

Spitz, DR ;

Yang, G ;

Tatarov, A ;

Lee, CS ;

Shegog, ML ;

Poss, KD .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (05) :1001-1011

[6] Expression and regulation of inducible nitric oxide synthase from human primary airway epithelial cells [J].

Donnelly, LE ;

Barnes, PJ .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2002, 26 (01) :144-151

[7] Expression of heme oxygenase in human airway epithelial cells [J].

Donnelly, LE ;

Barnes, PJ .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2001, 24 (03) :295-303

[8] Tobacco-smoke-inducible human haem oxygenase-1 gene expression: role of distinct transcription factors and reactive oxygen intermediates [J].

Favatier, F ;

Polla, BS .

BIOCHEMICAL JOURNAL, 2001, 353 :475-482

[9] Raised levels of exhaled carbon monoxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma:: a new marker of oxidative stress [J].

Horváth, I ;

Donnelly, LE ;

Kiss, A ;

Paredi, P ;

Kharitonov, SA ;

Barnes, PJ .

THORAX, 1998, 53 (08) :668-672

[10] OXIDANT STRESS LEADS TO TRANSCRIPTIONAL ACTIVATION OF THE HUMAN HEME OXYGENASE GENE IN CULTURED SKIN FIBROBLASTS [J].

KEYSE, SM ;

APPLEGATE, LA ;

TROMVOUKIS, Y ;

TYRRELL, RM .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (09) :4967-4969

← 1 2 3 →