A modified pGEX vector with a C-terminal histidine tag: Recombinant double-tagged protein obtained in greater yield and purity

被引：9

作者：

Strugnell, SA ^{[1
]}

Wiefling, BA ^{[1
]}

DeLuca, HF ^{[1
]}

机构：

[1] UNIV WISCONSIN, COLL AGR & LIFE SCI, DEPT BIOCHEM, MADISON, WI 53706 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 254卷 / 01期

关键词：

D O I：

10.1006/abio.1997.2425

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：147 / 149

页数：3

共 12 条

[1] Differential ligand-dependent interactions between the AF-2 activating domain of nuclear receptors and the putative transcriptional intermediary factors mSUG1 and TIF1 [J].

Baur, EV ;

Zechel, C ;

Heery, D ;

Heine, MJS ;

Garnier, JM ;

Vivat, V ;

LeDouarin, B ;

Gronemeyer, H ;

Chambon, P ;

Losson, R .

EMBO JOURNAL, 1996, 15 (01) :110-124

[2]

CHENG L, 1994, J BIOL CHEM, V269, P18662

[3] Synthesis and purification of soluble ligand binding domain of the human vitamin D-3 receptor [J].

Craig, TA ;

Kumar, R .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (03) :902-907

[4] SOLUBILIZATION AND PURIFICATION OF ENZYMATICALLY ACTIVE GLUTATHIONE-S-TRANSFERASE (PGEX) FUSION PROTEINS [J].

FRANGIONI, JV ;

NEEL, BG .

ANALYTICAL BIOCHEMISTRY, 1993, 210 (01) :179-187

[5] EUKARYOTIC PROTEINS EXPRESSED IN ESCHERICHIA-COLI - AN IMPROVED THROMBIN CLEAVAGE AND PURIFICATION PROCEDURE OF FUSION PROTEINS WITH GLUTATHIONE-S-TRANSFERASE [J].

GUAN, KL ;

DIXON, JE .

ANALYTICAL BIOCHEMISTRY, 1991, 192 (02) :262-267

[6] A CBP integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors [J].

Kamei, Y ;

Xu, L ;

Heinzel, T ;

Torchia, J ;

Kurokawa, R ;

Gloss, B ;

Lin, SC ;

Heyman, RA ;

Rose, DW ;

Glass, CK ;

Rosenfeld, MG .

CELL, 1996, 85 (03) :403-414

[7] NMR ASSIGNMENTS AND SECONDARY STRUCTURE OF THE RETINOID-X RECEPTOR-ALPHA DNA-BINDING DOMAIN - EVIDENCE FOR THE NOVEL C-TERMINAL HELIX [J].

LEE, MS ;

SEM, DS ;

KLIEWER, SA ;

PROVENCAL, J ;

EVANS, RM ;

WRIGHT, PE .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 224 (02) :639-650

[8] STRUCTURAL DETERMINANTS OF NUCLEAR RECEPTOR ASSEMBLY ON DNA DIRECT REPEATS [J].

RASTINEJAD, F ;

PERLMANN, T ;

EVANS, RM ;

SIGLER, PB .

NATURE, 1995, 375 (6528) :203-211

[9] OVERPRODUCTION OF RAT 1,25-DIHYDROXYVITAMIN-D3 RECEPTOR IN INSECT CELLS USING THE BACULOVIRUS EXPRESSION SYSTEM [J].

ROSS, TK ;

PRAHL, JM ;

DELUCA, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (15) :6555-6559

[10]

Schulman IG, 1996, MOL CELL BIOL, V16, P3807

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