Twenty-eightdays of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle

被引:49
作者
Jacobs, Robert A. [1 ,2 ,3 ]
Lundby, Anne-Kristine Meinild [1 ]
Fenk, Simone [1 ]
Gehrig, Saskia [1 ]
Siebenmann, Christoph [1 ,4 ]
Fluck, Daniela [1 ]
Kirk, Niels [1 ]
Hilty, Matthias P. [5 ]
Lundby, Carsten [1 ]
机构
[1] Univ Zurich, Inst Physiol, Zurich Ctr Integrat Human Physiol, Zurich, Switzerland
[2] Western Carolina Univ, Sch Teaching & Learning, Hlth & Phys Educ, Cullowhee, NC 28723 USA
[3] Western Carolina Univ, Phys Therapy Dept, Cullowhee, NC 28723 USA
[4] Royal Inst Technol, Sch Technol & Hlth, Dept Environm Physiol, Solna, Sweden
[5] Univ Zurich Hosp, Intens Care Unit, Zurich, Switzerland
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2016年 / 594卷 / 05期
基金
瑞士国家科学基金会;
关键词
MESSENGER-RNA EXPRESSION; HIGH-ALTITUDE; OPERATION EVEREST; ENERGY-EXPENDITURE; PROTEIN-SYNTHESIS; OXYGEN-UPTAKE; HYPOXIA; ADAPTATIONS; EXERCISE; ACCLIMATIZATION;
D O I
10.1113/JP271118
中图分类号
Q189 [神经科学];
学科分类号
071006 [神经生物学];
摘要
The role of hypoxia on skeletal muscle mitochondria is controversial. Studies superimposing exercise training on hypoxic exposure demonstrate an increase in skeletal muscle mitochondrial volume density (Mito(VD)) over equivalent normoxic training. In contrast, reductions in both skeletal muscle mass and Mito(VD) have been reported following mountaineering expeditions. These observations may, however, be confounded by negative energy balance, which may obscure the results. Accordingly we sought to examine the effects of high altitude hypoxic exposure on mitochondrial characteristics, with emphasis on Mito(VD), while minimizing changes in energy balance. For this purpose, skeletal muscle biopsies were obtained from nine lowlanders at sea level (Pre) and following 7 and 28days of exposure to 3454m. Maximal ergometer power output, whole body weight and composition, leg lean mass and skeletal muscle fibre area all remained unchanged following the altitude exposure. Transmission electron microscopy determined that intermyofibrillar (IMF) Mito(VD) was augmented (P=0.028) by 11.59.2% from Pre (5.050.9%) to 28Days (5.610.04%). In contrast, there was no change in subsarcolemmal (SS) Mito(VD). As a result, total Mito(VD) (IMF+SS) was increased (P=0.031) from 6.201.5% at Pre to 6.621.4% at 28Days (7.89.3%). At the same time no changes in mass-specific respiratory capacities, mitochondrial protein or antioxidant content were found. This study demonstrates that skeletal muscle Mito(VD) may increase with 28days acclimation to 3454 m.
引用
收藏
页码:1151 / 1166
页数:16
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