Aminoglycoside array for the high-throughput analysis of small molecule-RNA interactions

被引：25

作者：

Bryan, MC

Wong, CH

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

TETRAHEDRON LETTERS | 2004年 / 45卷 / 18期

关键词：

microarray; aminoglycoside; carbohydrate; RNA;

D O I：

10.1016/j.tetlet.2004.03.035

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Aminoglycoside-based oligosaccharides were bound to microtiter plates using noncovalent display with hybridization confirmed by mass spectrometry. Recognition of the saccharides by a model of the prokaryotic 16S aminoacyl site of the ribosomal decoding region was then determined. Tobramycin binding constants that correlated with surface plasmon resonance values for the model were effectively measured and novel means for binding aminoglycoside-based members were discovered for application to future aminoglycoside libraries. (C) 2004 Elsevier Ltd. All rights reserved.

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收藏

页码：3639 / 3642

页数：4

相关论文

共 22 条

[1] Probing the specificity of aminoglycoside ribosomal RNA interactions with designed synthetic analogs [J].

Alper, PB ;

Hendrix, M ;

Sears, P ;

Wong, CH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (09) :1965-1978

[2] Saccharide display on microtiter plates [J].

Bryan, MC ;

Plettenburg, O ;

Sears, P ;

Rabuka, D ;

Wacowich-Sgarbi, S ;

Wong, CH .

CHEMISTRY & BIOLOGY, 2002, 9 (06) :713-720

[3]

Dejong Eric S., 2002, Current Topics in Medicinal Chemistry, V2, P289, DOI 10.2174/1568026023394245

[4] Synthesis of sugar arrays in microtiter plate [J].

Fazio, F ;

Bryan, MC ;

Blixt, O ;

Paulson, JC ;

Wong, CH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (48) :14397-14402

[5]

Gesteland R. F., 1999, RNA WORLD, P709

[6] Design and synthesis of new aminoglycoside antibiotics containing neamine as an optimal core structure: Correlation of antibiotic activity with in vitro inhibition of translation [J].

Greenberg, WA ;

Priestley, ES ;

Sears, PS ;

Alper, PB ;

Rosenbohm, C ;

Hendrix, M ;

Hung, SC ;

Wong, CH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (28) :6527-6541

[7] A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets [J].

Hamasaki, K ;

Rando, RR .

ANALYTICAL BIOCHEMISTRY, 1998, 261 (02) :183-190

[8] Aminoglycoside binding in the major groove of duplex RNA: The thermodynamic and electrostatic forces that govern recognition [J].

Jin, E ;

Katritch, V ;

Olson, WK ;

Kharatisvili, M ;

Abagyan, R ;

Pilch, DS .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 298 (01) :95-110

[9] Thermodynamics of aminoglycoside-rRNA recognition: The binding of neomycin-class aminoglycosides to the A site of 16S rRNA [J].

Kaul, M ;

Pilch, DS .

BIOCHEMISTRY, 2002, 41 (24) :7695-7706

[10] INTERACTION OF ANTIBIOTICS WITH FUNCTIONAL SITES IN 16S RIBOSOMAL-RNA [J].

MOAZED, D ;

NOLLER, HF .

NATURE, 1987, 327 (6121) :389-394