Cytokine production induced by a 67-kDa fimbrial protein from Porphyromonas gingivalis

被引:24
作者
Hamada, N [1 ]
Watanabe, K [1 ]
Arai, M [1 ]
Hiramine, H [1 ]
Umemoto, T [1 ]
机构
[1] Kanagawa Dent Coll, Dept Oral Microbiol, Yokosuka, Kanagawa 2388580, Japan
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 2002年 / 17卷 / 03期
关键词
fimbriae; inflammatory cytokine; Porphyromonas gingivalis;
D O I
10.1034/j.1399-302X.2002.170311.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Fimbriae have been reported to play an important role in the adherence of Porphyromonas gingivalis to oral surfaces and possibly in triggering host responses. P. gingivalis ATCC 33277 has two distinctly different fimbriae expressed on the cell surface. The 67-kDa fimbriae differ in size and antigenicity from the earlier reported FimA, a major 41-kDa fimbrial component of P. gingivalis. Expression of the 67-kDa fimbriae on the cell surface of a fimA mutant was investigated by electron microscopy. The 67-kDa fimbrial protein was purified from the fimA mutant by sonication, precipitation, and chromatography on a DEAE Sepharose CL-6B column. The N-terminal amino acid sequence of the 67-kDa fimbrillin was distinct from that of the 41-kDa fimbrillin. Moreover, we have found that the 67-kDa fimbrial protein from P. gingivalis ATCC 33277 induced IL-1alpha, IL-beta, IL-6 and TNFalpha cytokine expression in mouse peritoneal macrophages. These results suggest that P. gingivalis 67-kDa fimbriae may play a part in the inflammatory response during the development of periodontal diseases.
引用
收藏
页码:197 / 200
页数:4
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