Chromatographic determination of polysaccharides in Lycium barbarum Linnaeus

被引：129

作者：

Wang, Chia Chi ^{[1
]}

Chang, Shyh Chung ^{[1
]}

Chen, Bing Huei ^{[1
]}

机构：

[1] Fu Jen Catholic Univ, Dept Food Sci, Taipei 242, Taiwan

来源：

FOOD CHEMISTRY | 2009年 / 116卷 / 02期

关键词：

Polysaccharide; Lycium barbarum Linnaeus; High-performance size exclusion chromatography; Gas chromatography; NONSTARCH POLYSACCHARIDES; CHLORELLA-PYRENOIDOSA; CHEMICAL-COMPOSITION; ANTITUMOR-ACTIVITY; GANODERMA-LUCIDUM; PROTEIN COMPLEX; FRUIT; IDENTIFICATION; FLAVONOIDS; MS;

D O I：

10.1016/j.foodchem.2009.03.015

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Polysaccharides in Lycium barbarum Linnaeus have been shown to be effective in preventing cancer. The Objectives Of this study were to develop an appropriate method for molecular weight determination of polysaccharides in L. barbarum. The Most suitable analytical condition was: a volume-ratio of L. barbarum sample to deionized water at 1:10, followed by shaking in a 100 degrees C water bath for 30 min, concentrating to 50 nil. and adding 250 mL of 95% ethanol for precipitation at - 20 degrees C for, 8 h, hydrolysing protein with 2.5 U/ml of proteinase at pH 8 and 60 degrees C for 4 It and separating polysaccharide into five fractions by high-performance Size exclusion chromatography (HPSEC) with the molecular Weight Of two Major fractions being 79,250 and 24,468 Da. Analysis of monosaccharides by gas chromatography (GC) indicated the presence of rhamnose. arabinose, xylose, mannose, glucose and galactose, with the molar ratio at 0.3:2.7:0.3:0.2:27:0.9, respectively. (C) 2009 Elsevier, Ltd. All rights reserved.

引用

页码：595 / 603

页数：9

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