Comparative study on the determination of cephalexin in its dosage forms by spectrophotometry and HPLC with UV-vis detection

被引：20

作者：

CampinsFalco, P ^{[1
]}

SevillanoCabeza, A ^{[1
]}

GalloMartinez, L ^{[1
]}

BoschReig, F ^{[1
]}

MonzoMansanet, I ^{[1
]}

机构：

[1] UNIV VALENCIA,FAC QUIM,DEPT QUIM FIS,VALENCIA,SPAIN

来源：

MIKROCHIMICA ACTA | 1997年 / 126卷 / 3-4期

关键词：

cephalexin; intact drug; acid-degraded drug; spectrophotometry; H-point standard additions method;

D O I：

10.1007/BF01242322

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper discusses the spectrophotometric determination of cephalexin as the intact cephalexin or as its acid-induced degradation product. Cephalexin can be determined in the range 1 x 10(-5)-18 x 10(-5) M with relative standard deviations of 5-1%. The limits of quantitation and detection were 10(-5) and 0.3 x 10(-5) M, respectively. These procedures were compared with reversed-phase HPLC determination. No interference was observed in the presence of common pharmaceutical adjuvants. The H-point standard additions method was applied in order to correct for the possible presence of the cephalexin precursor, 7-aminocephalosporanic acid; this improves the selectivity of the UV-vis spectrophotometric method.

引用

页码：207 / 215

页数：9

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