Mayaro virus infection cycle relies on casein kinase 2 activity

被引：11

作者：

Barroso, MMS ^{[1
]}

Lima, CS ^{[1
]}

Silva-Neto, MAC ^{[1
]}

Da Poian, AT ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, CCS, ICB, Dept Bioquim Med, BR-21941590 Rio De Janeiro, Brazil

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 296卷 / 05期

关键词：

CK2; Mayaro virus; virus replication;

D O I：

10.1016/S0006-291X(02)02093-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replication of Mayaro virus in Vero cells induces dramatic cytopathic effects and cell death. In this study, we have evaluated the role of casein kinase 2 (CK2) during Mayaro virus infection cycle. We found that CK2 was activated during the initial stages of infection (similar to36% after 4 h). This activation was further confirmed when the enzyme was partially purified from the cellular lysate either by Mono Q 5/5 Hr column or heparin-agarose column. Using this later column, we found that the elution profile of CK2 activity from infected cells was different from that obtained for control cell enzyme, suggesting a structural modification of CK2 after infection. Treatment of infected cells with a cell-permeable inhibitor of CK2, dichloro-1-(beta-D-ribofuranosyl)benzimidazole (DRB), abolished the cytopathic effect in a dose-dependent manner. Together this set of data demonstrates for the first time that CK2 activity in host cells is required in Mayaro virus infection cycle. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：1334 / 1339

页数：6

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