Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells

被引：25

作者：

Akihiro, Takashi

Umezawa, Taishi

Ueki, Chie

Lobna, Ben Mohamed

Mizuno, Kouichi

Ohta, Masaru

Fujimura, Tatsuhito ^{[1
]}

机构：

[1] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan

[2] RIKEN Plant Sci Ctr, Gene Discovery Res Team, Tsukuba, Ibaraki 3050074, Japan

[3] Natl Agron Inst Tunisia, Tunis 1082, Tunisia

[4] Akita Prefectural Univ, Fac Bioresource Sci, Akita 0100195, Japan

来源：

FEBS LETTERS | 2006年 / 580卷 / 25期

关键词：

ABA; sucrose; AGPase; starch; cDNA-AFLP;

D O I：

10.1016/j.febslet.2006.09.065

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified 27 genes induced by combined sucrose and ABA treatment from rice cultured cells with cDNA-AFLP. Thirteen of these up-regulated genes were induced 30 min after the co-treatment. This suite of genes includes starch biosynthesis related genes. Type A genes were expressed only in the presence of both sucrose and ABA. Type B genes were expressed in the presence of sucrose or ABA and the expression was dramatically enhanced by the co-treatment of sucrose and ABA. These results indicate that multiple steps of starch biosynthesis and other processes may be regulated by at least two different pathways. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5947 / 5952

页数：6

共 24 条

[1] Gene expression of ADP-glucose pyrophosphorylase and starch contents in rice cultured cells are cooperatively regulated by sucrose and ABA [J].