Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp israelensis

被引:241
作者
Berry, C
O'Neil, S
Ben-Dov, E
Jones, AF
Murphy, L
Quail, MA
Holden, MTG
Harris, D
Zaritsky, A
Parkhill, J
机构
[1] Cardiff Univ, Cardiff Sch Biosci, Cardiff CF10 3US, S Glam, Wales
[2] Sanger Inst, Hinxton Cambridge CB10 1SA, England
[3] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
关键词
D O I
10.1128/AEM.68.10.5082-5095.2002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The entire 127,923-bp sequence of the toxin-encoding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis is presented and analyzed. In addition to the four known Cry and two known Cyt toxins, a third Cyt-type sequence was found with an additional C-terminal domain previously unseen in such proteins. Many plasmid-encoded genes could be involved in several functions other than toxin production. The most striking of these are several genes potentially affecting host sporulation and germination and a set of genes for the production and export of a peptide antibiotic.
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页码:5082 / 5095
页数:14
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