Real-time monitoring of circulating tumor cell release during tumor manipulation using in vivo photoacoustic and fluorescent flow cytometry

被引:76
作者
Juratli, Mazen A. [1 ,2 ]
Sarimollaoglu, Mustafa [1 ]
Siegel, Eric R. [3 ]
Nedosekin, Dmitry A. [1 ]
Galanzha, Ekaterina I. [1 ,4 ]
Suen, James Y. [4 ]
Zharov, Vladimir P. [1 ,4 ]
机构
[1] Univ Arkansas Med Sci, Arkansas Nanomed Ctr, Phillips Class Laser & Nanomed Labs, Little Rock, AR 72205 USA
[2] Univ Marburg, Dept Otolaryngol Head & Neck Surg, Marburg, Germany
[3] Univ Arkansas Med Sci, Dept Biostat, Little Rock, AR 72205 USA
[4] Univ Arkansas Med Sci, Dept Otolaryngol Head & Neck Surg, Little Rock, AR 72205 USA
来源
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2014年 / 36卷 / 08期
基金
美国国家卫生研究院;
关键词
circulating tumor cells; melanoma; breast cancer; in vivo flow cytometry; photoacoustic and fluorescence methods; METASTATIC BREAST-CANCER; PROGRESSION; SURVIVAL; BLOOD;
D O I
10.1002/hed.23439
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 [耳鼻咽喉科学];
摘要
Background. Circulating tumor cells (CTCs) form metastases in distant organs. The purpose of this research was to determine if tumor manipulation could enhance cancer cell release from the primary tumor into the circulatory system. Methods. Nude mice were inoculated with melanoma or breast cancer cells. The implanted tumor underwent compression, biopsy, complete resection, or laser treatment. CTCs were monitored in the bloodstream using in vivo photoacoustic and fluorescence flow cytometry. Results. We discovered that pressure, biopsy, and laser treatment can dramatically increase CTC counts (up to 60-fold), whereas proper tumor resection significantly decrease CTC counts. Conclusion. Standard medical procedures could trigger CTC release that may increase the risk of metastases. This finding suggests the guidance of cancer treatment and likely diagnosis by real-time monitoring of CTC dynamics followed by well-timed treatment to reduce CTCs in the blood. In vivo detection of intervention-amplified CTCs could be used for early diagnosis of a small tumor, which is undetectable with conventional methods. (C) 2013 Wiley Periodicals, Inc.
引用
收藏
页码:1207 / 1215
页数:9
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