Identification of a 5S rDNA spacer type specific to Triticum urartu and wheats containing the T-urartu genome

被引：10

作者：

Allaby, RG ^{[1
]}

Brown, TA ^{[1
]}

机构：

[1] Univ Manchester, Inst Sci & Technol, Dept Biomol Sci, Manchester M60 1QD, Lancs, England

来源：

GENOME | 2000年 / 43卷 / 02期

关键词：

5S rDNA; polymerase chain reaction; Triticum; wheat;

D O I：

10.1139/gen-43-2-250

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A PCR system was designed to amplify 5S spacer rDNA specifically from homeologous chromosome 1 in a variety of species representative of the Aegilops and Triticum genera. Two polymerase chain reaction (PCR) primer combinations were used, one of which appears to be apomorphic in nature and specific to chromosome 1A in Triticum urartu and tetraploid and hexaploid wheats containing the AA genome donated by T. urartu. The value of studying single repeat types to investigate the molecular evolution of 5S-rDNA arrays is considered.

引用

页码：250 / 254

页数：5

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