Metabolic Labeling of Sialic Acids in Living Animals with Alkynyl Sugars

被引：173

作者：

Chang, Pamela V. ^{[1
,2
,3
,4
]}

Chen, Xing ^{[1
,2
,3
,4
]}

Smyrniotis, Chris ^{[1
,2
,3
,4
]}

Xenakis, Alexander ^{[1
,2
,3
,4
]}

Hu, Tianshun ^{[5
]}

Bertozzi, Carolyn R. ^{[1
,2
,3
,4
]}

Wu, Peng ^{[5
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[4] Mol Foundry Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

[5] Yeshiva Univ, Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 22期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

alkynes; click chemistry; glycobiology; nonnatural sugars; sialic acids; CELL-SURFACES; CANCER; GLYCANS; GLYCOPROTEOMICS; CYCLOADDITION; VISUALIZATION; EXPRESSION; TN;

D O I：

10.1002/anie.200806319

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sialome sweet sialome: As sialic acids are involved in many host-pathogen recognition events and are markers of embryonic and malignant tissues, there is great interest in methods for the enrichment and identification of sialylated glycoproteins from complex tissues. Now N-(4-pentynoyl) mannosamine can be used to metabolically label sialylated glycoproteins in living animals, enabling future identification of new biomarkers. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4030 / 4033

页数：4

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