Transepithelial transport of HIV-1 by M cells is receptor-mediated

被引:75
作者
Fotopoulos, G
Harari, A
Michetti, P
Trono, D
Pantaleo, G
Kraehenbuhl, JP [1 ]
机构
[1] Univ Lausanne, Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland
[2] Univ Lausanne, Inst Biochem, CH-1066 Epalinges, Switzerland
[3] CHU Vaudois, Lab AIDS Immunopathogensis, Div Clin Immunol, CH-1011 Lausanne, Switzerland
[4] CHU Vaudois, Div Gastroenterol, CH-1011 Lausanne, Switzerland
[5] Univ Geneva, Fac Med, Dept Genet & Microbiol, CH-1211 Geneva, Switzerland
关键词
D O I
10.1073/pnas.142586899
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human colon carcinoma Caco-2 cell monolayers undergo conversion into cells that share morphological and functional features of M cells when allowed to interact with B lymphocytes. A lymphotropic (X4) HIV-1 strain crosses M cell monolayers and infects underlying CD4(+) target cells. Transport requires both lactosyl cerebroside and CXCR4 receptors, which are expressed on the apical surface of Caco-2 and M cells. Antibodies specific for each receptor block transport. In contrast, a monotropic (R5) HIV-1 strain is unable to cross M cell monolayers and infect underlying monocytes, despite efficient transport of latex beads. Caco-2 and M cells do not express CCR5, but transfection of these cells with CCR5 cDNA restores transport of R5 virus, which demonstrates that HIV-1 transport across M cells is receptor-mediated. The follicle-associated epithelium covering human gut lymphoid follicles expresses CCR5, but not CXCR4, and lactosyl cerebroside, suggesting that HIV-1 infection may occur through M cells and enterocytes at these sites.
引用
收藏
页码:9410 / 9414
页数:5
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