Encapsulation of Multiple Biological Compounds Within a Single Electrospun Fiber

The encapsulation of multiple biological compounds within a single electrospun fiber to focus on therapeutic applications of electrospun fibers for drug, gene or protein delivery in tissue engineering was reported. The experiment involved two model proteins, BSA fluorescent labeled with Texas-Red (BSA-TR) and epidermal growth factor which was encapsulated in nanoparticles and introduced into electrospun biocompatible PU fibers. The fabrication of these multiprotein loaded electrospun fiber includes two distinct processes: nanoparticle formation using a single-emulsion method and electrospinning. The study compared the encapsulation of a single component inside an electrospun fiber thereby offering the opportunity to control the release of multiple compounds, potentially at distinct rates with an aim of attaining controlled, distinct release profiles of multiple proteins from a single electrospun fiber.