Determination of Organic Acids in Tissues and Exudates of Maize, Lupin, and Chickpea by High-Performance Liquid Chromatography-Tandem Mass Spectrometry

被引：24

作者：

Erro, Javier ^{[1
]}

Zamarreno, Angel M. ^{[2
]}

Yvin, Jean-Claude ^{[2
]}

Garcia-Mina, Jose M. ^{[1
,2
]}

机构：

[1] Univ Navarra, Dept Chem & Soil Chem, E-31080 Pamplona, Spain

[2] CIPAV Poligono Arazuri Orcoyen, R&D AFI Roullier Grp, Orcoyen 31160, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 10期

关键词：

ESI-MS/MS; ion-exclusion chromatography; organic acids determination; maize; lupin; chickpea; ION-EXCLUSION CHROMATOGRAPHY; ALIPHATIC CARBOXYLIC-ACIDS; GAS-CHROMATOGRAPHY; CAPILLARY-ELECTROPHORESIS; ELECTROCHEMICAL DETECTION; DICARBOXYLIC-ACIDS; RHIZOSPHERE; MOBILIZATION; SEPARATION; QUANTIFICATION;

D O I：

10.1021/jf804003v

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

This article describes a fast and simple methodology for the extraction and determination of organic acids in tissues and root exudates of maize, lupin, and chickpea by LC/MS/MS. Its main advantage is that it does not require sample prepurification before HPLC analysis or sample derivatization to improve sensibility. The results obtained showed good precision and accuracy, a recovery close to 100%, and no significant matrix effect. Moreover, the sensibility of the method is in general better than that of previously described methodologies, with detection limits between 15 and 900 pg injected.

引用

页码：4004 / 4010

页数：7

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