Glucose oxidase - An overview

被引：1288

作者：

Bankar, Sandip B. ^{[1
]}

Bule, Mahesh V. ^{[1
]}

Singhal, Rekha S. ^{[1
]}

Ananthanarayan, Laxmi ^{[1
]}

机构：

[1] Univ Mumbai, Inst Chem Technol, Food Engn & Technol Dept, Bombay 400019, Maharashtra, India

来源：

BIOTECHNOLOGY ADVANCES | 2009年 / 27卷 / 04期

关键词：

Glucose oxidase; Catalse; Optimization of fermentation parameters; Downstream processing; Immobilization; Application in biosensors; PENICILLIUM-VARIABILE P16; ASPERGILLUS-NIGER; SACCHAROMYCES-CEREVISIAE; HYDROGEN-PEROXIDE; METAL-IONS; CATALASE; ENZYME; PURIFICATION; AMAGASAKIENSE; EXPRESSION;

D O I：

10.1016/j.biotechadv.2009.04.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Glucose oxidase (beta-D-glucose:oxygen 1 -oxidoreductase; EC 1.1.2.3.4) catalyzes the oxidation of beta-D-glucose to gluconic acid, by utilizing molecular oxygen as an electron acceptor with simultaneous production of hydrogen peroxide. Microbial glucose oxidase is currently receiving Much attention due to its wide applications in chemical, pharmaceutical, food, beverage, clinical chemistry, biotechnology and other industries. Novel applications of glucose oxidase in biosensors have increased the demand in recent years. Present review discusses the production, recovery, characterization, immobilization and applications of glucose oxidase. Production of glucose oxidase by fermentation is detailed, along with recombinant methods. Various purification techniques for higher recovery of glucose oxidase are described here. Issues of enzyme kinetics, stability studies and characterization are addressed. Immobilized preparations Of glucose oxidase are also discussed. Applications of glucose oxidase in various industries and as analytical enzymes are having an increasing impact on bioprocessing. (c) 2009 Elsevier Inc. All rights reserved

引用

页码：489 / 501

页数：13

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