The human tap nuclear RNA export factor contains a novel transportin-dependent nuclear localizatoin signal that lacks nuclear export signal function

被引:54
作者
Truant, R
Kang, YB
Cullen, BR [1 ]
机构
[1] Duke Univ, Med Ctr, Howard Hughes Med Inst, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Dept Genet, Durham, NC 27710 USA
关键词
D O I
10.1074/jbc.274.45.32167
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human Tap protein mediates the sequence specific nuclear export of RNAs containing the constitutive transport element and is likely also critical for general mRNA export. Here, we demonstrate that a previously defined arginine-rich nuclear localization signal (NLS) present in Tap acts exclusively via the transportin import factor. Previously, transportin has been shown to mediate the nuclear import of several heterogeneous nuclear ribonucleoproteins, including heterogeneous nuclear ribonucleoprotein (hnRNP) Al, by binding to a sequence element termed M9. Although the Tap NLS and the hnRNP Al M9 element are shown to compete for transportin binding, they show no sequence homology, and the Tap NLS does not conform to the recently defined M9 consensus. The Tap NLS also differs from M9 in that only the latter is able to act as a nuclear export signal. The Tap NLS is therefore the first member of a novel class of transportin-specific NLSs that lack nuclear export signal function.
引用
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页码:32167 / 32171
页数:5
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