Virus-Specific Antibody Secreting Cell, Memory B-cell, and Sero-Antibody Responses in the Human Influenza Challenge Model

被引:46
作者
Huang, Kuan-Ying Arthur [1 ,2 ]
Li, Chris Ka-Fai [1 ]
Clutterbuck, Elizabeth [3 ,4 ]
Chui, Cecilia [1 ]
Wilkinson, Tom [5 ]
Gilbert, Anthony [6 ]
Oxford, John [6 ]
Lambkin-Williams, Rob [6 ]
Lin, Tzou-Yien [2 ,7 ]
McMichael, Andrew J. [1 ]
Xu, Xiao-Ning [1 ]
机构
[1] Univ Oxford, Weatherall Inst Mol Med, Human Immunol Unit, Oxford OX3 9DS, England
[2] Chang Gung Childrens Hosp, Dept Pediat, Taoyuan, Taiwan
[3] Univ Oxford, Ctr Clin Vaccinol & Trop Med, Oxford Vaccine Grp, Oxford OX1 2JD, England
[4] Univ Oxford, Ctr Clin Vaccinol & Trop Med, NIHR Oxford Biomed Res Ctr, Oxford OX1 2JD, England
[5] Univ Southampton, Fac Med, Southampton SO9 5NH, Hants, England
[6] Retroscreen Virol, London, England
[7] Chang Gung Univ, Coll Med, Guishan Township, Taiwan
基金
英国医学研究理事会;
关键词
B cells; human challenge model; influenza A virus; PLASMA-CELLS; IMMUNE-RESPONSE; INFECTION; VACCINE; PROTECTION; IMMUNIZATION; INDIVIDUALS; ACTIVATION; CHILDREN; ELISPOT;
D O I
10.1093/infdis/jit650
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Antibodies play a major role in the protection against influenza virus in human. However, the antibody level is usually short-lived and the cellular mechanisms underlying influenza virus-specific antibody response to acute infection remain unclear. Methods. We studied the kinetics and magnitude of influenza virus-specific B-cell and serum antibody responses in relation to virus replication during the course of influenza infection in healthy adult volunteers who were previously seronegative and experimentally infected with seasonal influenza H1N1 A/Brisbane/59/07 virus. Results. Our data demonstrated a robust expansion of the virus-specific antibody-secreting cells (ASCs) and memory B cells in the peripheral blood, which correlated with both the throat viral load and the duration of viral shedding. The ASC response was obviously detected on day 7 post-infection when the virus was completely cleared from nasal samples, and serum hemagglutination-inhibition antibodies were still undetectable. On day 28 postinfection, influenza virus-specific B cells were further identified from the circulating compartment of isotype-switched B cells. Conclusions. Virus-specific ASCs could be the earliest marker of B-cell response to a new flu virus infection, such as H7N9 in humans.
引用
收藏
页码:1354 / 1361
页数:8
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