The developments of semisynthetic DNA-protein conjugates

被引:148
作者
Niemeyer, CM [1 ]
机构
[1] Univ Dortmund, Fachbereich Chem, D-44227 Dortmund, Germany
关键词
D O I
10.1016/S0167-7799(02)02022-X
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Semisynthetic DNA-protein conjugates, generated by either covalent or non-covalent coupling chemistry, are versatile molecular tools applicable in bioanalytical and synthetic chemical procedures. This article reviews the synthesis and characterization of artificial nucleic acid-protein conjugates, in addition to applications arising in the life sciences and nanobiotechnology, such as the self-assembly of high-affinity reagents for immunological detection assays and biosensors, the fabrication of laterally microstructured biochips, and the biomimetic 'bottom-up' synthesis of nanostructured supramolecular devices.
引用
收藏
页码:395 / 401
页数:7
相关论文
共 83 条
[1]   Engineering polydactyl zinc-finger transcription factors [J].
Beerli, RR ;
Barbas, CF .
NATURE BIOTECHNOLOGY, 2002, 20 (02) :135-141
[2]   Changing functionality of surfaces by directed self-assembly using oligonucleotides -: The Oligo-Tag [J].
Bier, FF ;
Kleinjung, F ;
Ehrentreich-Förster, E ;
Scheller, FW .
BIOTECHNIQUES, 1999, 27 (04) :752-+
[3]   Antibodies in diagnostics - from immunoassays to protein chips [J].
Borrebaeck, CAK .
IMMUNOLOGY TODAY, 2000, 21 (08) :379-382
[4]   Quantifying DNA-protein interactions by double-stranded DNA arrays [J].
Bulyk, ML ;
Gentalen, E ;
Lockhart, DJ ;
Church, GM .
NATURE BIOTECHNOLOGY, 1999, 17 (06) :573-577
[5]   48000-FOLD ACCELERATION OF HYBRIDIZATION BY CHEMICALLY-MODIFIED OLIGONUCLEOTIDES [J].
COREY, DR .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (36) :9373-9374
[6]   GENERATION OF A HYBRID SEQUENCE-SPECIFIC SINGLE-STRANDED DEOXYRIBONUCLEASE [J].
COREY, DR ;
SCHULTZ, PG .
SCIENCE, 1987, 238 (4832) :1401-1403
[7]   Antibody arrays for high-throughput screening of antibody-antigen interactions [J].
de Wildt, RMT ;
Mundy, CR ;
Gorick, BD ;
Tomlinson, IM .
NATURE BIOTECHNOLOGY, 2000, 18 (09) :989-994
[8]  
Demers LM, 2001, ANGEW CHEM INT EDIT, V40, P3071, DOI 10.1002/1521-3773(20010817)40:16<3071::AID-ANIE3071>3.0.CO
[9]  
2-S
[10]   MOLECULAR ENGINEERING - AN APPROACH TO THE DEVELOPMENT OF GENERAL CAPABILITIES FOR MOLECULAR MANIPULATION [J].
DREXLER, KE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-PHYSICAL SCIENCES, 1981, 78 (09) :5275-5278