An examination of the binding of aluminum to protein and mineral components of bone and teeth

被引:7
作者
Rowatt, E
Sorensen, ES
Triffit, J
Viess, A
Williams, RJP
机构
[1] UNIV OXFORD, INORGAN CHEM LAB, OXFORD OX1 3QR, ENGLAND
[2] UCL, SCH MED, DEPT MED MICROBIOL, LONDON W1N 8AA, ENGLAND
[3] UNIV AARHUS, DEPT BIOL MOL, PROT CHEM LAB, AARHUS C, DENMARK
[4] NUFFIELD ORTHOPAED CTR, BONE RES LAB, MRC, OXFORD, ENGLAND
[5] NORTHWESTERN UNIV, DEPT BASIC SCI, CHICAGO, IL 60611 USA
关键词
D O I
10.1016/S0162-0134(97)00088-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Aluminum binding by a variety of noncollagenous proteins associated with bone and teeth (osteopontin, osteocalcin and phosphophoryn) and by hydroxyapatite are examined. The proteins bound aluminum with a dissociation constant, K-D, of the 10(-7) M or greater at pH = 7. The number of atoms of aluminum bound was found to be related to bat not equivalent to the number of phosphorylated serines in osteopontin and phosphophoryn. Osteocalcin bound one aluminum tightly presumably due to its gamma-carboxyl glutamate residues. Hydroxyapatite bound Al3+ tightly releasing 1.5 equivalent Ca2+ per Al3+ bound. Addition of 3 mM Ca2+, close to the total concentration found in animal circulating fluids, did not effect noticeably the amount of Al3+ bound to bone which mast have a K-D for Al3+ < 10(-6). Silicic acid added after equilibration With all these materials has little effect bat neither the proteins nor hydroxyapatite removed much Al3+ from pre-equilibrated Al3+ solution with silicic acid. The results are discussed with regard to Al3+ poisoning. (C) 1997 Elsevier Science Inc.
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页码:235 / 238
页数:4
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