cDNA cloning and expression of the Xenopus laevis vitellogenin receptor

A Xenopus laevis oocyte cDNA library was screened with a PCR-generated X, laevis vitellogenin (VTG) receptor probe and a 3.6 kb cDNA clone containing the entire open reading frame, and 5' and 3' noncoding regions were isolated. The deduced amino acid sequence was 72% homologous to the chicken VLD/VTG receptor, and the characteristic domains were highly conserved. Ligand binding analysis confirmed that the cloned receptor was Xenopus VTG-specific, Although Northern blotting analysis revealed that this gene was expressed as a major transcript of 3.6 kb in Xenopus ovary, weak hut significant expression was observed in other tissues by RT-PCR analysis. The fact that major expression of the gene occurs in the ovary suggests that it has an important function in this organ. (C) 1996 Academic Press, Inc.