Quantification of mitochondrial DNA copy number: Pre-analytical factors

被引：110

作者：

Andreu, Antonio L. ^{[2
]}

Martinez, Ramiro ^{[2
]}

Marti, Ramon ^{[2
]}

Garcia-Arumi, Elena ^{[1
,2
]}

机构：

[1] Univ Vall Hebron, Inst Rec Hosp, CIBBIM, Unitat Patol Mitocondrial,Lab 104, Barcelona 08035, Spain

[2] ISCIII, CIBERER, Barcelona, Spain

来源：

MITOCHONDRION | 2009年 / 9卷 / 04期

关键词：

mtDNA depletion syndrome; Mitochondrial DNA; Real-time PCR; DNA isolation and purification methods; BLOOD MONONUCLEAR-CELLS; REAL-TIME PCR; SKELETAL-MUSCLE; DEPLETION; MTDNA; LEUKOCYTES; DIAGNOSIS; INCREASE; TISSUE;

D O I：

10.1016/j.mito.2009.02.006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Mitochondrial DNA (mtDNA) content is important for understanding many cellular processes. Several pre-analytical factors, from sample collection to DNA extraction can affect measurement of mtDNA copy number. In the present study, whole blood samples yielded a higher mtDNA copy number than buffy coat samples. mtDNA content is affected by the cell separation method used and the time between blood withdrawal and cell separation. Thus, reference values must be established with the same type of sample. As to the DNA isolation and purification method, the manual phenol method can give randomly false high values. The QIAamp DNA Mini Kit provided the most highly reproducible mtDNA/nDNA yield. (C) 2009 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：242 / 246

页数：5

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