In vivo degradation of 14C-labeled porcine dermis biologic scaffold

被引:46
作者
Carey, Lisa E. [1 ,2 ]
Dearth, Christopher L. [1 ,3 ]
Johnson, Scott A. [2 ]
Londono, Ricardo [2 ,4 ]
Medberry, Christopher J. [1 ,2 ]
Daly, Kerry A. [1 ,3 ,5 ]
Badylak, Stephen E. [1 ,2 ,3 ]
机构
[1] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15219 USA
[2] Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA
[3] Univ Pittsburgh, Dept Surg, Pittsburgh, PA 15213 USA
[4] Univ Pittsburgh, Med Scientist Training Program, Pittsburgh, PA 15261 USA
[5] Dept Agr, Canberra, ACT 2601, Australia
关键词
Extracellular matrix; Dermis; Biomaterial degradation; C-14; quantification; SMALL-INTESTINAL SUBMUCOSA; XENOGENEIC EXTRACELLULAR-MATRIX; MACROPHAGE PHENOTYPE; COLLAGEN-FIBERS; WALL REPAIR; ARRANGEMENT; SKIN; REGENERATION; ARCHITECTURE; OUTCOMES;
D O I
10.1016/j.biomaterials.2014.06.015
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Biologic scaffold materials are used for repair and reconstruction of injured or missing tissues. Such materials are often composed of allogeneic or xenogeneic extracellular matrix (ECM) manufactured by decellularization of source tissue, such as dermis. Dermal ECM (D-ECM) has been observed to degrade and remodel in vivo more slowly than other biologic scaffold materials, such as small intestinal submucosa (SIS-ECM). Histologic examination is a common method for evaluating material degradation, but it lacks sensitivity and is subject to observer bias. Utilization of C-14-proline labeled ECM is a quantitative alternative for measuring degradation of ECM scaffolds. Using both methods, the amount of degradation of D-ECM and SIS-ECM was determined at 2, 4, and 24 weeks post-implantation in a rodent model. Results utilizing C-14 liquid scintillation counting (LSC) analysis showed distinct differences in degradation at the three time points. D-ECM material in situ stayed the same at 76% remaining from 2 to 4 weeks post-implantation, and then decreased to 44% remaining at 24 weeks. In the same time period, implanted SIS-ECM material decreased from 72% to 13% to 0%. Visual examination of device degradation by histology overestimated degradation at 2 weeks and underestimated device degradation at 24 weeks, compared to the C-14 method. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:8297 / 8304
页数:8
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