Characterization of the 3a protein of SARS-associated coronavirus in infected Vero e6 cells and SARS patients

被引：76

作者：

Zeng, R

Yang, RF

Shi, MD

Jiang, MR

Xie, YH

Ruan, HQ

Jiang, XS

Shi, L

Zhou, H

Zhang, L

Wu, XD

Lin, Y

Ji, YY

Xiong, L

Jin, Y

Dai, EH

Wang, XY

Si, BY

Wang, J

Wang, HX

Wang, CE

Gan, YH

Li, YC

Cao, JT

Zuo, JP

Shan, SF

Xie, E

Chen, SH

Jiang, ZQ

Zhang, X

Wang, Y

Pei, G

Sun, B

Wu, JR

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Proteome Anal, Key Lab Proteon, Shanghai 200031, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Shanghai 200031, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China

[4] Acad Mil Med Sci, Inst Microbial & Epidemiol, Beijing 100071, Peoples R China

[5] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China

[6] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Laboratorial Anim Ctr, Shanghai 201615, Peoples R China

[7] Chinese Acad Sci, Shanghai Inst Biol Sci, Cell Resources Ctr, Cell Bank Type Culture Collect Comm, Shanghai 200031, Peoples R China

[8] Shanghai Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2004年 / 341卷 / 01期

基金：

中国国家自然科学基金;

关键词：

SARS; coronavirus; 3a protein; spike protein; proteome;

D O I：

10.1016/j.jmb.2004.06.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：271 / 279

页数：9

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